puncta selection script (matlab) Search Results


plugin  (Oxford Instruments)
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Oxford Instruments plugin
Plugin, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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puncta selection script (matlab)  (MathWorks Inc)
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MathWorks Inc puncta selection script (matlab)
Puncta Selection Script (Matlab), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab function regionprops  (MathWorks Inc)
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MathWorks Inc matlab function regionprops
Matlab Function Regionprops, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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puncta measurement script (matlab)  (MathWorks Inc)
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MathWorks Inc puncta measurement script (matlab)

Puncta Measurement Script (Matlab), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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code for colocalized synaptic puncta in astrocyte territories  (MathWorks Inc)
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MathWorks Inc code for colocalized synaptic puncta in astrocyte territories
(A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized <t>puncta</t> density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .
Code For Colocalized Synaptic Puncta In Astrocyte Territories, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alphashape function  (MathWorks Inc)
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MathWorks Inc alphashape function
(A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized <t>puncta</t> density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .
Alphashape Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automated puncta analysis  (MathWorks Inc)
90
MathWorks Inc automated puncta analysis
(A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized <t>puncta</t> density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .
Automated Puncta Analysis, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: eLife

Article Title: Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation

doi: 10.7554/eLife.45107

Figure Lengend Snippet:

Article Snippet: Software, algorithm , Puncta measurement script (MATLAB) , , PMID: 30661800 , , .

Techniques: Knock-In, Phospho-proteomics, Control, Construct, Dominant Negative Mutation, Affinity Purification, Western Blot, Software, Membrane

(A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized puncta density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .

Journal: Cell reports

Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

doi: 10.1016/j.celrep.2022.110416

Figure Lengend Snippet: (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized puncta density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .

Article Snippet: MATLAB code for colocalized synaptic puncta in astrocyte territories , This manuscript , https://doi.org/10.5281/zenodo.5879992.

Techniques: Immunostaining

(A) Schematic of PALE with CMV-shRNA-tGFP and pCAG-mTdT in postnatal mice. (B) P21 PALE astrocytes from deep layers of scramble-shRNA and Lrig1-shRNA groups. Scale bar, 10 μm. (C) Sholl analysis of scramble-shRNA and Lrig1-shRNA astrocytes. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; each group includes 26–35 cells (z stack). (D and E) Immunostaining for VGluT1/PSD95 (D) and VGluT2/PSD95 (E) excitatory synapses in P21 scr-shRNA and Sparc-shRNA groups. Scale bar, 10 μm. (F and G) Quantification of co-localized puncta density in tdTomato + astrocyte domains. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; Sparc-shRNA: n = 6 mice; Il33-shRNA: n = 7 mice; each V1 group includes 29–33 cells (z stack) and each V2 group includes 23–26 cells. Data represent mean ± SEM; statistical analyses were multiple t test (C) or one-way ANOVA (F, G). See also .

Journal: Cell reports

Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

doi: 10.1016/j.celrep.2022.110416

Figure Lengend Snippet: (A) Schematic of PALE with CMV-shRNA-tGFP and pCAG-mTdT in postnatal mice. (B) P21 PALE astrocytes from deep layers of scramble-shRNA and Lrig1-shRNA groups. Scale bar, 10 μm. (C) Sholl analysis of scramble-shRNA and Lrig1-shRNA astrocytes. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; each group includes 26–35 cells (z stack). (D and E) Immunostaining for VGluT1/PSD95 (D) and VGluT2/PSD95 (E) excitatory synapses in P21 scr-shRNA and Sparc-shRNA groups. Scale bar, 10 μm. (F and G) Quantification of co-localized puncta density in tdTomato + astrocyte domains. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; Sparc-shRNA: n = 6 mice; Il33-shRNA: n = 7 mice; each V1 group includes 29–33 cells (z stack) and each V2 group includes 23–26 cells. Data represent mean ± SEM; statistical analyses were multiple t test (C) or one-way ANOVA (F, G). See also .

Article Snippet: MATLAB code for colocalized synaptic puncta in astrocyte territories , This manuscript , https://doi.org/10.5281/zenodo.5879992.

Techniques: shRNA, Immunostaining

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

doi: 10.1016/j.celrep.2022.110416

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MATLAB code for colocalized synaptic puncta in astrocyte territories , This manuscript , https://doi.org/10.5281/zenodo.5879992.

Techniques: Recombinant, RNAscope, Multiplex Assay, Real-time Polymerase Chain Reaction, In Situ Hybridization, Software